Sodium chlorite-Dossier: Sodium chlorite-Short-term toxicity to fish-001

http://www.qsartoolbox.org/de/web/guest/registration-dossier/-/registered-dossier/2233/6/2/2/?documentUUID=4fb54c5c-2c63-43e8-91c7-b727a2162498

Sodium chlorite

EC number: 231-836-6 | CAS number: 7758-19-2

Short-term toxicity to fish-001

Administrative data

Purpose flag:

key study

Study result type:

experimental result

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

The test substance could not be monitored in the test solutions due to background interference. However, the method allowed accurate determination of the stock solution concentrations in deionised water. As a flow-through system was used, the nominals are considered acceptable estimates of the actual concentration.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Materials and methods

Test guideline

Qualifier:

according to

Guideline:

EPA OPP 72-1 (Fish Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):

yes

Test material identityopen allclose all

Test material identity 1

Identifier:

CAS number

Identity:

7758-19-2

Test material identity 2

Identifier:

EC number

Identity:

231-836-6

Test material identity 3

Identifier:

IUPAC name

Identity:

sodium chlorite

Details on test material:

Lot/Batch number: 09-M, PAL M-033, used for screening toxicity test; 75-A/PAL A-300, used for the definitive test
Purity: 80%
Further relevant properties: A white powder

Analytical monitoring:

yes

Details on sampling:

Sampling: Dissolved oxygen, pH, salinity, and temperature were measured daily in each test chamber that contained live animals. The test temperature in one test vessel was recorded continuously during the test.

Monitoring of TS concentration: No, the test substance was not monitored throughout the test as background interference prevented it.

Vehicle:

no data

Test organisms

Test organisms (species):

Cyprinodon variegatus

Study design

Test type:

flow-through

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

22.7 - 23 °C

pH:

7.6 - 8.2

Dissolved oxygen:

7.0 - 7.8 mg/L

Nominal and measured concentrations:

Nominal concentrations of the active ingredient were 0 mg/L (control), 22.5, 37.5, 60, 90 and 150 mg sodium chlorite/L. Test concentrations were adjusted for the purity of the test substance.
(These doses are equivalent to 0, 17, 28, 45, 67 and 112 mg chlorite/L)

Reference substance (positive control):

no

Results and discussion

Effect concentrationsopen allclose all

Effect concentrations 1

Duration:

96 h

Endpoint:

LC0

Effect conc.:

60 other: mg sodium chlorite/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

96 h

Endpoint:

LC50

Effect conc.:

105 other: mg sodium chlorite/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks (e.g. 95% CL):

95 % confidence interval of 90 to 150 mg/L

Effect concentrations 3

Duration:

96

Endpoint:

LC50

Effect conc.:

78 other: mg clorite/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 4

Duration:

96 h

Endpoint:

NOEC

Effect conc.:

60 other: mg sodium chlorite/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 5

Duration:

96 h

Endpoint:

NOEC

Effect conc.:

45 other: mg chlorite/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

At a nominal concentration of 150 mg/L some fish were lethargic at 48 h. Exposure of Sheepshead minnows to the test substance resulted in a 96 h LC50 of 105 mg/L sodium chlorite with a 95 % confidence interval of 90 to 150 mg/L (equivalent to 78 mg chlorite/L). The 96 h NOEC was 60 mg/L sodium chlorite (equivalent to 45 mg chlorite/L).

Any other information on results incl. tables:

Table A7_4_1_1(2)-5:        Mortality data

Test-Substance Concentration (nominal) [mg sodium chlorite/L]	Mortality
	Number     24 h          48 h          72 h          96 h				Percentage     24 h         48 h          72 h           96 h
0 (control)	0	0	0	0	0	0	0	0
	0	0	0	0	0	0	0	0
22.5	0	0	0	0	0	0	0	0
	0	0	0	0	0	0	0	0
32.7	0	0	0	1	0	0	0	5
	0	0	0	0	0	0	0	0
60	0	0	0	1	0	0	0	5
	0	0	0	0	0	0	0	0
90	0	0	1	2	0	0	5	10
	0	1	1	2	0	5	5	10
150	0	9	10	10	0	90	100	100
	0	8	10	10	0	80	100	100

Table A7_4_1_1(2)-6:        Sub-lethal effect data

Test-Substance Concentration (nominal) [mg sodium chlorite/L]	Lethargy
	Number     24 h          48 h          72 h          96 h				Percentage     24 h         48 h          72 h           96 h
0 (control)	0	0	0	0	0	0	0	0
	0	0	0	0	0	0	0	0
22.5	0	0	0	0	0	0	0	0
	0	0	0	0	0	0	0	0
32.7	0	0	0	0	0	0	0	0
	0	0	0	0	0	0	0	0
60	0	0	0	0	0	0	0	0
	0	0	0	0	0	0	0	0
90	0	0	0	0	0	0	0	0
	0	0	0	0	0	0	0	0
150	0	1	-	-	0	5	-	-
	0	2	-	-	0	10	-	-

Table A7_4_1_1(2)-7:        Effect data

	96 h [mg/l]1	95 % c. l. [mg/l]
LC0	60	Not specified
LC50	105 (n)	90 to 150
LC100	Not specified	Not specified

1 indicate effect data are based on nominal (n) concentrations

Applicant's summary and conclusion

Conclusions:

96 h LC50 = 105 mg/L sodium chlorite with a 95 % confidence interval of 90 to 150 mg/L (equivalent to 78 mg chlorite/L).

Executive summary:

The aim of this study was to investigate the short-term toxicity to fish produced by the test material.

The test procedure used was the EPA OPP 72-1 (Fish Acute Toxicity Test).

The result was as follows:

96 h LC50 = 105 mg/L sodium chlorite with a 95 % confidence interval of 90 to 150 mg/L (equivalent to 78 mg chlorite/L).

Validity criteria fulfilled:

yes

Remarks:

(mortality in control was 0%; constant conditions were maintained; the dissolved oxygen concentration was within the acceptable range; the concentrations were maintained using a flow-through system).