Chlorine-Dioxide-Dossier-Sodium Chlorate-Short-term toxicity to fish.005

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Chlorine dioxide-Dossier

SodiumChlorate

• Short-term toxicity to fish.005

Short-term toxicity to fish.005

Administrative data

Purpose flag:

key study

Study result type:

read-across from supporting substance (structural analogue or surrogate)

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

Study generated according to valid and
internationally accepted testing guideline and performed
under GLP.
No chemical analyses were prformed on the test solution, only the stock solution could be analyzed. Because of analytical results from chronic tests it can be assumed that the test concentrations were stable and the fish were exposed properly.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report Date:

1991

Materials and methods

Test guideline

Qualifier:

according to

Guideline:

EPA OPP 72-1 (Fish Acute Toxicity Test)

GLP compliance:

yes

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):

no

Test material identityopen allclose all

Test material identity 1

Test material identity 2

Test material identity 3

Details on test material:

Purity: >99%

Analytical monitoring:

no

Details on sampling:

Analysis conducted on stock only.

Vehicle:

no

Details on test solutions:

Preparation: 600,000 mg/L stock solution was prepared by adding 1200.0 g test substance to 2000 ml deionized water. Appropriate amounts of stock were added directly to dilution water by a proportional diluter. The dilution water was filtered natural groundwater collected at Hampton, New Hampshire.

Test organisms

Test organisms (species):

Oncorhynchus mykiss

Details on test organisms:

Supplier: Aquatic Research Organisms, Hampton, New Hampshire
- Wild caught: No
- Age/weight/loading: juvenile, 0.57 g on average, 0.38 g/L
- Feeding: fish food (EnviroSystems lot number TM02) once or twice daily
- Pretreatment: acclimatized for 14 days under test conditions.
- Feeding during test: no, feeding stopped 48 hours prior to the test

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Pretreatment: acclimatized for 14 days under test conditions

Test conditions

Test temperature:

11.0-11.6 °C

pH:

6.8-7.3

Dissolved oxygen:

9.0-9.8 mg/L

Nominal and measured concentrations:

0 mg/L (control), 150 1 240, 380, 600, and 1,000 mg/L. (Nominal)

Details on test conditions:

- Test type: flow-through, 9.0 media exchanges per 24 hours in each test vessel.
- Exposure vessel type: 20 L glass aquaria that contained 15 L of test solution
- Number of replicates, fish per replicate: 2, 10
- Conductivity: 490-1200 µmhos/cm (equal to µS/cm) at the start of the test and 530-680 µmhos/cm (equal to µS/cm) at the end
- Intensity of irradiation: cool white fluorescent lights with an intensity of 12 µEs/m2
- Photoperiod: 16 hours photoperiod daily
- Nominal test concentrations: 0, 150, 240, 380, 600, 1000 mg/L. Measured concentration of the stock solution was 630,000 mg/L

Dilution water:
– Source: groundwater collected from wells in Hampton, New Hampshire
- Aeration: Yes
- Hardness: 48 mg CaCO3/L
- pH: 6.8
- Conductance: 490 µmhos/cm (equal to µS/cm)
- Holding water: same as dilution water

Reference substance (positive control):

no

Results and discussion

Effect concentrationsopen allclose all

Effect concentrations 1

Effect concentrations 2

Details on results:

mortality and loss of equilibrium, erratic swimming, loss of reflex, excitability, discoloration or change in behavior.
- Mortality: At 72 hours 1 fish was dead in 1000 mg/l
- Affected: At 72 hours 2 and 4 fish were lethargic in the 2 replicates of 1000 mg/l. At 96 hours only 1 and 2 fish respectively showed these effects
in the same concentration.
- No effects were observed in the control, all fish were normal.

Results with reference substance (positive control):

Not Reported

Reported statistics and error estimates:

No statistics could be performed because greater than 50% survival occurred in all test concentrations.

Applicant's summary and conclusion

Conclusions:

Study performed to EPA guidelines under flow through conditions with GLP accreditation. Stocks were analysed but analysis in the test media was not possible to interference. The stock recovery was 105%. Due to the test substance stability and the fact that the solution is continually renewed and
providing the automatic diluting system used was working accurately the nominal concentrations can be considered reliable. The LC50 of
>1000mg/l is considered reliable without major restrictions.

Executive summary:

Exposure of rainbow trout to the test substance resulted in a 96 hour LC50 greater than 1,000 mg/L sodium chlorate. The 96 hour no observed effect concentration was 600 mg/L sodium chlorate.

Validity criteria fulfilled:

no

Remarks:

The test concentrations could not be monitored, only the stock solution was analyzed.

EC number: 233-162-8 | CAS number: 10049-04-4

Short-term toxicity to fish.005