Chlorine Dioxide Dossier:Chlorine Dioxide-Short-term toxicity to fish-002

http://www.qsartoolbox.org/de/web/guest/registration-dossier/-/registered-dossier/15450/6/2/2/?documentUUID=0cf1ccc5-fd6e-4094-9f07-83c1d171f8e0

Chlorine dioxide

EC number: 233-162-8 | CAS number: 10049-04-4

Short-term toxicity to fish

Currently viewing: Exp WoE Short-term toxicity to fish.008Read-across Subs Key Short-term toxicity to fish.012NS Supporting Short-term toxicity to fish.007Exp Supporting Short-term toxicity to fish.001Read-across Subs Key Short-term toxicity to fish.010Exp Supporting Short-term toxicity to fish.006Exp WoE Short-term toxicity to fish.009Exp Key Short-term toxicity to fish.003Exp Supporting Short-term toxicity to fish.002Read-across Subs Key Short-term toxicity to fish.005NS Supporting Short-term toxicity to fish.011Exp Supporting Short-term toxicity to fish.004Read-across Subs Key Short-term toxicity to fish.014NS Supporting Short-term toxicity to fish.013 

• Administrative data
• Data source
• Materials and methods
• Results and discussion
• Applicant's summary and conclusion

Some information in this page has been claimed confidential.

Administrative data

Purpose flag:

supporting study

Study result type:

experimental result

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

No guideline or GLP compliance stated

Data source

Reference

Reference Type:

publication

Title:

Comparison of chlorine and chlorine dioxide toxicity to fathead minnows and bluegill.

Author:

Wilde EW, Soracco JR Mayack LA, Shealy RL, Broadwell TL and Steffen RF

Year:

1983

Bibliographic source:

Water Resources, 17(10): 1327-1331

Materials and methods

Principles of method if other than guideline:

Flow-through 96 hour bioassays were conducted on juvenile and adult fathead minnows using a mobile laboratory located adjacent to Par Pond, to test ClO2 toxicity. Dissolved oxygen, temperature, conductivity and pH in the test chambers were measured in selected chambers.

GLP compliance:

no data

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):

yes

Test material identityopen allclose all

Test material identity 1

Test material identity 2

Analytical monitoring:

yes

Details on sampling:

Measurements of dissolved oxygen, temperature, conductivity and pH in the test chambers receiving 100, 32, 5.6 and 0% doses of the b iocide stock solutions were made at the beginning of the tests and at 24 hour intervals during the tests (Hydrolab 8000 was used).

Details on test solutions:

ClO2 solutions were prepared by adding previously standardised mixture of NaOCl, HCl and sodium chlorite. Stock solutions were added to their respective dilutor systems for 1 hour at approximately 0, 24, 48 and 72 hours into the testing period. Two stock solutions were prepared 30 minutes apart, 15 minutes prior to their use during thedosing period. One was used during the first half hour and the other during the remainder of the dosing period, a procedure used to compensate for the degradation of the solutions. 100, 56, 32,18, 5.6 and 0% of stock solution of the biocide was delivered to duplicate test chambers using Par Pond as a dilutent. The dilutor systems were adjusted to cycle every 1.8 min resulting in 22.9 volume changes per day in each chamber. However, during the daily biocide exposure periods, and one hour thereafter, systems were adjusted to a cycle time of 1.5 in to achieve one complete volume change an hour.

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

* Juvenile fathead minnows - age: 4 weeks old; feed: brine shrimp (Artemia sp.) daily during acclimation and test periods to prevent starvation
* Adult fathead minnows - age: 1 year old; feed: not fed

Fish were acclimated to Par Pond water for 3 days prior to the initiation of the bioassays. During the first 24 h the fish were slowlly acclimated from well water (approx. 22oC, pH8.2) to Par Pond water (approx 28oC, pH 7.0).

Study design

Test type:

flow-through

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Test temperature:

Mean ± S.D.: 27.7 ± 0.2oC
Range: 24.5 - 30.4oC

pH:

Mean ± S.D.: 7.0 ± 0.1
Range: 6.7 - 7.5

Dissolved oxygen:

Mean ± S.D.: 6.8 ± 0.1 mg/L
Range: 3.8 - 7.9 mg/L

Details on test conditions:

CONDUCTIVITY:
Mean ± S.D.: 69.9 ± 0.3 µmhos/cm
Range: 64 - 81 µmhos/cm

Results and discussion

Effect concentrationsopen allclose all

Effect concentrations 1

Effect concentrations 2

Details on results:

In mg/L:
* Juvenile fathead minnows
96-h peak: 0.07 (0.06 - 0.08)
96-h mean max: 0.06 (0.05 - 0.07)
96-h mean: 0.02 (0.01 - 0.02)
96-h accum.: 3.26 (2.10 - 4.54)
(Chi-square is small: P > 0.10)

* Adult fathead minnows
96-h peak: 0.63 (0.33 - 2.74)
96-h mean max: 0.46 (0.25 - 1.61)
96-h mean: 0.17 (0.13 - 0.24)
96-h accum.: 93.47 (40.97 - 327.83)

Reported statistics and error estimates:

Median lethal concentrations (96-h LC50s) were determined by probit analysis. Log transformations of dose values were not used , because a bettter goodness of fit was obtained in the majority of cases by using actual dose values. Computations were performed using an IBM computer and a Statistical Analysis System (SAS) program. Biocide doses were calculated as follows:
(1) 96-h peak = the single highest biocide residual level detected during the tests
(2) 96-h mean maximum = the average maximum biocide residual detected during the 4 days of testing
(3) 96-h mean = the mean biocide residual level during the four approx. 2 h exposure periods.
(4) 96-h accumulative exposure = the total 96-h biocide exposure in mg/L residual x min of exposure (area under a time-concnetration curve).

Applicant's summary and conclusion