Chlorine dioxide-Dossier: Chlorine Dioxide-Short-term toxicity to fish.012

http://www.qsartoolbox.org/de/web/guest/registration-dossier/-/registered-dossier/15450/6/2/2/?documentUUID=7d1cb0bb-56f6-417f-8cde-f24fb9084459

Chlorine dioxide-Dossier: Chlorine Dioxide

• Short-term toxicity to fish.012

Short-term toxicity to fish.012

Administrative data

Purpose flag:

key study

Study result type:

read-across from supporting substance (structural analogue or surrogate)

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

Study generated according to generally valid and
internationally accepted testing guideline and performed
under GLP.
No analytical monitoring was performed, only the stock solution was analyzed, but based on chronic tests it can be assumed that the test substance was stable during the test and the fish were properly exposed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report Date:

1991

Materials and methods

Test guideline

Qualifier:

according to

Guideline:

other guideline: EPA-FIFRA, guideline 72-3

Deviations:

no

GLP compliance:

yes

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):

no

Test material identityopen allclose all

Test material identity 1

Test material identity 2

Test material identity 3

Details on test material:

Purity: >99.0%

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

- Preparation: 600,000 mg/L stock solution was prepared by adding 1200.0 g test substance to 2000 ml deionized water. Appropriate amounts of stock were added directly to dilution water by a proportional diluter.

Test organisms

Test organisms (species):

Cyprinodon variegatus

Details on test organisms:

- Supplier: The aquatic research organisms division of resource analysts, Inc., Hampton, New Hampshire 
- Wild caught: No 
- Age/weight/loading: juvenile 
- Feeding: fish food (EnviroSystems lot number TM02) once or twice daily 
- Pretreatment: acclimatized for more than 7 days under test conditions. 
- Feeding during test: no, feeding stopped 48 hours prior to the test

Study design

Test type:

flow-through

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21.8-22.9 °C

pH:

7.6-7.8

Dissolved oxygen:

7.4-8.1 mg/L

Salinity:

15-17 ppt

Nominal and measured concentrations:

Nominal concentrations: 0, 140, 240, 380, 600, 1000 mg/L 

Details on test conditions:

DILUTION WATER: 
- Source: unfiltered sea water collected from Atlantic ocean in Hampton, New Hampshire 
- Aeration: Yes 
- Salinity: 16 ppt (parts per thousand) 
- pH: 7.6 
- Holding water: same as dilution water 

TEST SYSTEM: 
- Test type: 7.8 media exchanges per 24 hours in each test vessel. 
- Exposure vessel type: 20 L glass aquaria that contained 15 L of test solution 
- Number of replicates, fish per replicate: 2, 10 
- Intensity of irradiation: cool white fluorescent lights with an intensity of 12 µEs/m2 
- Photoperiod: 16 hours photoperiod daily

Reference substance (positive control):

no

Results and discussion

Effect concentrationsopen allclose all

Effect concentrations 1

Effect concentrations 2

Details on results:

Measured concentration of the stock solution was 600,000 mg/L

Effect data (Mortality): In the control and in all test concentrations no mortality and no sublethal effects were observed.

Reported statistics and error estimates:

No statistics could be performed because greater than 50% survival occurred in all test concentrations.

Applicant's summary and conclusion

Conclusions:

only the stock solution could be analyzed, but based on chronic tests for which chemical analyses were performed it can be assumed that the test concentrations were stable and that the fish were exposed properly. No fish died during the test, therefore the 96h-LC50 is greater than 1000 mg/l and the NOEC is 1000 mg/l.

Executive summary:

The acute toxicity of sodium chlorate to the sheepshead minnow, Cyprinodon variegatus, is described in this final report. The test

was conducted for Albright and Wilson Americas for 96 hours during February 19 to 23, 1991, at the EnviroSystems Division of Resource Analysts, Inc. in Hampton, New Hampshire. It was conducted by Peter Kowalski, Ellen Stanford, Jeanne Magazu, Robert Boeri, and Timothy Ward according to the protocol developed for EnviroSystems Study Number 90115-DE. The analytical portion of this study was conducted under the supervision of Gloria Switalski. Sodium chlorate (reported purity >99% active ingredient) was supplied by the sponsor.

The test was performed under flow-through conditions with five concentrations of test substance and a dilution water control at a temperature of 22 ± 1°C. The dilution water was unfiltered natural seawater collected at Hampton, New Hampshire. Mean nominal concentrations of sodium chlorate were: 0 mg/L (control), 140, 240, 380, 600, and 1,000 mg/L. Nominal concentrations were used for all calculations.

Organism used in the test were procured from a commercial supplier (Aquatic Research Organisms, Hampton, New Hampshire) and acclimated at EnviroSystems under test conditions for more than 7 days. After 96 hours of exposure the control organisms had an average wet weight (blotted dry) of 0.70 g. All animals were in good condition at the beginning of the study.

Exposure of sheepshead minnow to the test substance resulted in a 96 hour LC50 greater than 1,000 mg/L sodium chlorate. The 96 hour no observed effect concentration was 1,000 mg/L sodium chlorate.

Validity criteria fulfilled:

no

Remarks:

the test substance concentration could not be monitored

EC number: 233-162-8 | CAS number: 10049-04-4

Short-term toxicity to fish.012