Chlorine dioxide-Dossier:Chlorine Dioxide-Short-term toxicity to fish.009

http://www.qsartoolbox.org/de/web/guest/registration-dossier/-/registered-dossier/15450/6/2/2/?documentUUID=953c9e19-8be4-41fb-8612-a23d4521f0f9

Chlorine dioxide

EC number: 233-162-8 | CAS number: 10049-04-4

Short-term toxicity to fish.009

Administrative data

Purpose flag:

weight of evidence

Study result type:

experimental result

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report Date:

2005

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Methods by Dr Dinnel Shannon Pt. Marine Center

GLP compliance:

no data

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):

yes

Test material identity

Identifier:

CAS number

Identity:

10049-04-4

Details on test material:

- Name of test material (as cited in study report): Chlorine dioxide
- Molecular formula (if other than submission substance): no data
- Molecular weight (if other than submission substance): no data
- Smiles notation (if other than submission substance): no data
- InChl (if other than submission substance): no data
- Structural formula attached as image file (if other than submission substance): no data
- Substance type: powder
- Physical state: solid dissolved in deionized water
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: no data
- Isomers composition: no data
- Purity test date: no data
- Lot/batch No.: no data
- Expiration date of the lot/batch: no data
- Radiochemical purity (if radiolabelling): not applicable
- Specific activity (if radiolabelling): not applicable
- Locations of the label (if radiolabelling): not applicable
- Expiration date of radiochemical substance (if radiolabelling): not applicable
- Stability under test conditions: no data
- Storage condition of test material: no data
- Other: none

Details on properties of test surrogate or analogue material:

No data

Analytical monitoring:

no

Details on sampling:

- Concentrations: 0, 0.313, 0.625, 1.25, 2.5, and 5.0 mg/L
- Sampling method: test concentrations were verified at the beginning of the test using HACH DPD Method 10126
- Sample storage conditions before analysis: no data

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was made from powered product supplied by Ecochlor. Two separate powders were mixed according to directions by dissolving them in deionized water and bringing the volume to one liter to obtain a target concentration of 3000 ppm chlorine dioxide.
- Eluate: deionized water
- Differential loading: 0.5 X dilution series
- Controls: only seawater
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no data

Test organisms

Test organisms (species):

other: Clupea pallasi

Details on test organisms:

TEST ORGANISM
- Common name: Pacific herring
- Strain: no data
- Source: P. Hershberger, U.S. Geological Survey
- Age at study initiation (mean and range, SD): < 24 h post hatch
- Length at study initiation (length definition, mean, range and SD): no data
- Weight at study initiation (mean and range, SD): no data
- Method of breeding: no data
- Feeding during test NO
- Food type: none
- Amount: none
- Frequency: none

ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): no data
- Type and amount of food: no data
- Feeding frequency: no data
- Health during acclimation (any mortality observed): no data

QUARANTINE (wild caught)
- Duration: no data
- Health/mortality: no data

Study design

Test type:

other: static with renewal at 48 h

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

no data

Test conditions

Hardness:

no data

Test temperature:

10+-1°C

pH:

no data

Dissolved oxygen:

no data

Salinity:

16+-1 ppt

Nominal and measured concentrations:

- nominal conc.: 0, 0.313, 0.625, 1.25, 2.5, 5.0 mg/L
- measured conc.: -, -, -, -, 1.8, 3.6 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 300 mL glass beaker
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 150 mL
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter): none
- Renewal rate of test solution (frequency/flow rate): renewal at 48 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 10
- Biomass loading rate: no data

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater diluted with deionized water
- Total organic carbon: no data
- Particulate matter: no data
- Metals: no data
- Pesticides: no data 
- Chlorine: no data
- Alkalinity: no data
- Ca/mg ratio: no data 
- Conductivity: no data
- Culture medium different from test medium: not applicable
- Intervals of water quality measurement: no data

OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: 16:8
- Light intensity: no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 0.5 X dilution
- Justification for using less concentrations than requested by guideline: no data
- Range finding study 
- Test concentrations: 0, 0.313, 0.625, 1.25, 2.5, 5.0 mg/L 
- Results used to determine the conditions for the definitive study: no data

Reference substance (positive control):

yes

Remarks:

Sodium Dodecyl Sulfate

Results and discussion

Effect concentrations

Duration:

96 h

Endpoint:

LC50

Effect conc.:

ca. 0.35 mg/L

Details on results:

no data

Results with reference substance (positive control):

- Results with reference substance valid
- Mortality: EC50(96h) = 6.9 mg/L of SDS

Reported statistics and error estimates:

no data

Any other information on results incl. tables:

Table: Results (mean +- standard deviation) of the 96h acute test using C. pallasi.

Chlorine dioxide (mg/L)			Survival (%)
Nominal	Measured
0	-		95.0 ± 5.8
0.313	-		95.0 ± 5.8
0.625	-		10.0 ± 8.1
1.25	-		0
2.5	1.8		0
5	3.6		0

Applicant's summary and conclusion

Conclusions:

Toxicity of the substance has been tested on C. pallasi, in a 96h acute test. The results demonstrated that a LC50(96h) = 0.35 mg/L.

Executive summary:

Toxicity of the substance has been tested on Clupea pallasi, in a 96 h acute-renewal test. A control and five different concentrations (0.313, 0.625, 1.25, 2.5, and 5.0 mg/L) were tested in 4 replicates, each replicate containing 10 individuals. The solution was completely changed every days of the experiment. The results demonstrated a LC50(96h) = 0.35 mg/L of chlorine dioxide.

Validity criteria fulfilled:

no data